Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Encapsulated Probiotic Candy With Freeze-Dry Method
DOI:
https://doi.org/10.24235/sc.educatia.v11i1.10159Keywords:
Candy, Encapsulan, Freeze dry, Probiotic, ViabilityAbstract
The level of viable cell count in probiotic candy, based on WHO standard is >106 CFU/ml or >106 CFU/g. Meanwhile, information on the bacterial viability of probiotic candy according to WHO standards is still limited. The experimental study was conducted to discover the viability of Lactobacillus bulgaricus and Streptococcus thermophilus in encapsulated probiotic candy. Encapsulants consisted of maltodextrin, gum, corn starch, and skim milk. Encapsulation of probiotic bacteria used freeze-dry method. Probiotic candies were stored at room temperature for 14 days in an aerobic condition. The viability of probiotics candy was tested and the results showed significance for each type of encapsulation (p<0.05). The gum and corn starch encapsulations showed bacteria viability that met WHO standards for functional foods, which ranged from 20,333,333±7,637,626to 31,553,333±2,741,894 CFU/g (>106 CFU/g). The most preferred encapsulant in terms of taste, texture, and aroma was identified as skim milk encapsulation. Further research on the long-term storage of probiotic candy and the viability of probiotic candy bacteria in the gastrointestinal tract (in vivo) is needed.References
Adib, A., H. Wahid, M., Sudarmono, P., & S. Surono, I. (2013). Lactobacillus plantarum Pada Feses Individu Dewasa Sehat yang Mengkonsumsi Lactobacillus plantarum IS-10506 dari Dadih. Jurnal Teknologi Dan Industri Pangan, 24(2), 154–161. https://doi.org/10.6066/jtip.2013.24.2.154
Amin, T., Thakur, M., & Jain, S. C. (2013). Microencapsulation-the Future of Probiotic Cultures. 3(1), 35–43.
Bilang, M., Tahir, M., & Haedar, D. (2018). Mempelajari Viabilitas Enkapsulasi Sel Probiotik (Lactobacillus plantarum dan Streptococcus thermophilus) Pada Es Krim. Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 41–52. https://doi.org/10.20956/canrea.v1i1.21
Calinoiu, L. F., Ştefanescu, B. E., Pop, I. D., Muntean, L., & Vodnar, D. C. (2019). Chitosan coating applications in probiotic microencapsulation. Coatings, 9(3), 1–21. https://doi.org/10.3390/COATINGS9030194
Diza, Y. H., Asben, A., & Anggraini, T. (2020). Isolasi, Identifikasi, dan Penyiapan Sediaan Kering Asam Laktat yang Berpotensi sebagai Probiotik dari Dadih asal sijungjung Sumatera Barat. Jurnal Litbang Industri, 10(2), 155–164.
Etchepare, M. de A., Raddatz, G. C., Cichoski, A. J., Flores, É. M. M., Barin, J. S., Queiroz Zepka, L., Jacob-Lopes, E., Grosso, C. R. F., & de Menezes, C. R. (2016). Effect of resistant starch (Hi-maize) on the survival of Lactobacillus acidophilus microencapsulated with sodium alginate. Journal of Functional Foods, 21, 321–329. https://doi.org/10.1016/j.jff.2015.12.025
Firmansyah, A. (2016). Terapi Probiotik dan Prebiotik pada Penyakit Saluran Cerna Anak. Sari Pediatri, 2(4), 210. https://doi.org/10.14238/sp2.4.2001.210-4
Fransiska, T. R., & Djaenudin, D. (2021). Enkapsulasi Probiotik Lactobacillus sp. Menggunakan Dua Tahap Proses. Jurnal Serambi Engineering, 6(2), 1832–1841. https://doi.org/10.32672/jse.v6i2.2883
Ghasemnezhad, R., Razavilar, V., Pourjafar, H., Khosravi-Darani, K., & Ala, K. (2017). The viability of free and encapsulated Lactobacillus casei and Bifidobacterium animalis in chocolate milk, and evaluation of its pH changes and sensory properties during storage. Annual Research and Review in Biology, 21(3), 1–8. https://doi.org/10.9734/ARRB/2017/37885
Haffner, F. B., Diab, R., & Pasc, A. (2016). Encapsulation of probiotics: Insights into academic and industrial approaches. AIMS Materials Science, 3(1), 114–136. https://doi.org/10.3934/matersci.2016.1.114
Hariyadi, P. (2013). Freeze Drying Technology :for Better Quality & Flavor of Dried Products. Foodreview Indonesia, VIII(2), 52–57.
Himawan, A., Prihatiningsih, T., & Hardini, N. (2018). Menurunkan Indeks Plak Dan Jumlah Koloni Streptococcus Sp . Saliva. 7(1), 1–10.
Hossain, M. N., Ranadheera, C. S., Fang, Z., Hutchinson, G., & Ajlouni, S. (2021). Protecting the viability of encapsulated Lactobacillus rhamnosus LGG using chocolate as a carrier. Emirates Journal of Food and Agriculture, 33(8), 647–656. https://doi.org/10.9755/ejfa.2021.v33.i8.2740
Kamil, R. Z., Yanti, R., Murdiati, A., Juffrie, M., & Rahayu, E. S. (2020). Microencapsulation of indigenous probiotic Lactobacillus plantarum dad-13 by spray and freeze-drying: Strain-dependent effect and its antibacterial property. Food Research, 4(6), 2181–2189. https://doi.org/10.26656/fr.2017.4(6).280
Karimi, R., Mortazavian, A. M., & Da Cruz, A. G. (2011). Viability of probiotic microorganisms in cheese during production and storage: A review. Dairy Science and Technology, 91(3), 283–308. https://doi.org/10.1007/s13594-011-0005-x
Khalighi, A., Behdani, R., & Kouhestani, S. (2016). Probiotics: A Comprehensive Review of Their Classification, Mode of Action and Role in Human Nutrition. Probiotics and Prebiotics in Human Nutrition and Health, 1–11. https://doi.org/10.5772/63646
Krasaekoopt, W., Bhandari, B., & Deeth, H. (2003). Evaluation of encapsulation techniques of probiotics for yoghurt. International Dairy Journal, 13(1), 3–13. https://doi.org/10.1016/S0958-6946(02)00155-3
Lestari, L. A., Kusuma, W. I., Nurhayati, F., Kusuma, R. J., & Erwanto, Y. (2020). The Viability of Probiotic Lactobacillus acidophilus ifo 13951 and Bifidobacterium longum atcc 15707 in Gummy Candies Decreased During 4 Weeks of Storage. Food Research, 4(4), 1191–1195. https://doi.org/10.26656/FR.2017.4(4).078
Okarini, I. A., & Suartiningsih, N. P. M. (2017). Susu sebagai Bahan Pangan Kimia, Mikrobiologi, Manfaat, Penanganan Susu dan Limbah. Jurnal Teknik Industri, 4(2), 75–89. https://simdos.unud.ac.id/uploads/file_penelitian_1_dir/b88eb5fafa765a8ae940bfa1da3144f4.pdf
Osmand, Purwijantiningsih, L. M., & Pranata, F. S. (2012). Viabilitas Bakteri dan Kualitas Permen Probiotik dengan Variasi Jenis Enkapsulan. 1–15.
Pavli, F., Tassou, C., Nychas, G. J. E., & Chorianopoulos, N. (2018). Probiotic incorporation in edible films and coatings: Bioactive solution for functional foods. International Journal of Molecular Sciences, 19(1). https://doi.org/10.3390/ijms19010150
Pradipta, M. S. I. (2017). Pengaruh Mikroenkapsulasi Probiotik Bakteri Asam Laktat Indigenous Unggas Menggunakan Bahan Penyalut Maltodekstrin Terhadap Viabilitas Selama Penyimpanan. Journal of Livestock Science and Production, 1(1), 37–43. https://doi.org/10.31002/jalspro.v1i1.452
Pratama, M. V., Meitiniarti, V. I., & Sukmana, A. B. A. (2019). Uji viabilitas bakteri asam laktat dalam enkapsulasi menggunakan alginat dan Susu skim secara kering dingin. Simposium Nasional Ilmiah Dengan Tema: (Peningkatan Kualitas Publikasi Ilmiah Melalui Hasil Riset Dan Pengabdian Kepada Masyarakat), November, 484–495. https://doi.org/10.30998/simponi.v0i0.483
Puspawati, N. N., Nuraida, L., & Adawiyah, D. R. (2010). Penggunaan berbagai jenis bahan pelindung untuk mempertahankan viabilitas bakteri asam laktat yang diisolasi dari air susu ibu pda proses pengeringan beku. Jurnal Teknologi Dan Industri Pangan, XXI(1), 59–65. https://journal.ipb.ac.id/index.php/jtip/article/view/2463
Rahmiati, R., & Mumpuni, M. (2017). Eksplorasi Bakteri Asam Laktat Kandidat Probiotik Dan Potensinya Dalam Menghambat Bakteri Patogen. Elkawnie, 3(2), 141–150. https://doi.org/10.22373/ekw.v3i2.1870
Samedi, L., & Charles, A. L. (2019). Viability of 4 probiotic bacteria microencapsulated with arrowroot starch in the simulated gastrointestinal tract (GIT) and yoghurt. Foods, 8(5). https://doi.org/10.3390/foods8050175
Sarkar, S. (2020). Spray Drying Encapsulation of Probiotics for Functional Food Formulation-A Review. Novel Techniques in Nutrition & Food Science, 5(2), 441–449. https://doi.org/10.31031/ntnf.2020.05.000610
Silaban, B. J. S., Nurhayati, L., & Hartanti, A. W. (2020). Viabilitas Probiotik Lactobacillus acidophilus DLBSD102 Setelah Mikroencapsulasi. Jurnal Sains Natural, 10(1), 6. https://doi.org/10.31938/jsn.v10i1.266
Sumanti, D. M., Lanti, I., Hanidah, I.-I., Sukarminah, E., & Giovanni, A. (2016). Pengaruh Konsentrasi Susu Skim dan Maltodekstrin Sebagai Penyalut Terhadap Viabilitas dan Karakteristik Mikroenkapsulasi Suspensi Bakteri Lactobacillus plantarum menggunakan metode freeze drying. Jurnal Penelitian Pangan (Indonesian Journal of Food Research), 1(1), 7–13. https://doi.org/10.24198/jp2.2016.vol1.1.02
Sunaryanto, R. (2017). Pengaruh Kombinasi Bakteri Asam Laktat Terhadap Perubahan Karakteristik Nutrisi Susu Kerbau. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 4(1), 21. https://doi.org/10.29122/jbbi.v4i1.2064
Suryani, S., Nofiandi, D., Mukhtar, H., Siska, M., Dharma, A., & Nasir, N. (2017). Identifikasi Molekular Bakteri Asam Laktat Lactobacillus paracasei yang ada pada Lapisan Minyak VCO. Jurnal Katalisator, 2(2), 79. https://doi.org/10.22216/jk.v2i2.2517
Triana, E., Yulianto, E., & Nurhidayat, N. (2006). Viability of encapsulated Lactobacillus sp. Mar 8. Biodiversitas Journal of Biological Diversity, 7(2), 114–117. https://doi.org/10.13057/biodiv/d070204
Trimudita, R. F. (2021). Enkapsulasi Probiotik Lactobacillus sp . Menggunakan. Serambi Engeneering, VI(2), 1832–1841.
Widyadnyana, D. G. A., Sukrama, I. D. M., & Suardana, I. W. (2015). Studies on Lactic Acid Bacteria Isolate 9A from Bali Cattle Colon as a Probiotic Through 16S rRNA gene Analysis. Indonesian Journal of Veterinary Science, 33(2), 228–233.
Yansyah, N., Studi, P., Hasil, T., Teknologi, J., Pertanian, H., Pertanian, F., & Riau, U. (2016). Yoghurt yang Dipermentasi Dengan Issolat Lactobacillus plantarum 1. Teknologi Hasil Pertanian, 3(2), 1–15.
Yulimatussa, A. P., Blambangan, B. G. P. B., Dewi, J. C., Herdianto, R. S., Nafiis, M. M., Rosyidah, I., Sutanti, T. N. E., Syarofi, N. M. R., Farmasi, F., & Airlangga, U. (2016). Pengetahuan Penanganan Halitosis Dalam Masalah Kesehatan Mulut. Jurnal Farmasi Komunitas, 3(2), 29.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Scientiae Educatia: Jurnal Pendidikan Sains

This work is licensed under a Creative Commons Attribution 4.0 International License.
LETTER OF COPYRIGHT AGREEMENT
I am the author of an article on Scientiae Educatia: Jurnal Pendidikan Sains in collaboration with the Perkumpulan Pendidik IPA Indonesia (PPII), the undersigned:
State that:
- My paper is the work itself, authentic, has never been published in journals and other publications;
- My paper is a work on original ideas / research, not the result of plagiarism;
- My paper was not published from the help of others, except on the recommendation of the editorial and reviewer board that was selected by this journal;
- My paper, there are no writings or opinions that I have written other than including the reference source in the bibliography;
- I give all my copyright of this paper to this journal include the right for managing and publication;
- I make this assignment surely. If there are distortion and untruth in this assignment, later, I will take responsibility as the current law.