The Utilization of Coconut Water Waste As a Growth Media of the In-Vitro Potato Cutting
DOI:
https://doi.org/10.24235/sc.educatia.v7i2.2636Keywords:
coconut water waste, in vitro, Solanum tuberosum L.Abstract
The limited number of potato seed caused the demand  The demand to be unfulfilled especially in South Sulawesi. The use of pathogen-free seeds is absolutely necessary that can be obtained through tissue culture. This study aims to determine the effect of adding coconut water on the growth of in vitro potato plant cuttings and the most effective type of treatment. This research used Varying Concentration of the coconut water, then measure its effect on potato growth (a high number of leaves and plantlets). This study consisted of 4 treatments: TQ0 (without coconut milk), TQ1 (100 ml/l), TQ2 (150ml/l), and TQ3 (200 ml/l). The results showed that the addition of coconut water on the growth of micro cuttings of potato (Solanum tuberosum L.) gave significant effect on the observed parameters, leaf number and plantlets weight. While TQ2 gave the best effect on the growth of in vitro micro cutting of potato.References
Abrahamian, P., and Kantharajah, A. (2011). Effect of vitamins on in vitro organogenesis of plant. American Journal of Plant Sciences, 2: 669-674. DOI: 10.4236/ajps.2011.25080
Aladele, S.E., and Kuta, D. D. (2008). Environmental and genotypic effects on the growth rate of in vitro cassava plantlet (Manihot esculenta Crantz). Afr. J. Biotechnol, 7(4): 381-385
Al-Khayri, J. M. (2001). Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.). In Vitro Cellular & Developmental Biology-Plant, 37(4): 453- 456. DOI: 10.1007/s11627-001-0079-x
Asaad, M., Aliem, B., Warda., Nasrullah., Tahir, H., Dawson, P. (2005). AGB/2005/167 Farmer initiated learning - potatoes South Sulawesi. Australian Centre for International Agriculture Research. Available online: http://aciar.gov.au/
Baque, M. A., Shin, Y. K., Elshmari, T., Lee, E. J., Paek, K. Y. (2011).
Effect of light quality, sucrose and coconut water concentration on the microporpagation of Calanthe hybrids ('Bukduseong' 'Hyesung' and 'Chunkwang' 'Hyesung'). Australian Journal of Crop Science, 5(1): 1247-1254
Bey, Y., Syafii, W., Ngafifah, N. (2005). Pengaruh pemberian giberelin pada media vacin dan went terhadap perkecambahan biji anggrek bulan (Phalaenopsis amabilis BL.) secara in vitro. Jurnal Biogenesis, 1(4): 57-60.
Fiorani, F., Beemster, G. T. S., Bultynck, L., Lambers, H. (2000). Can meristematic activity determine variation in leaf size and elongation rate among four Poa species? a kinematic study. Plant Physiol, 124 (2): 485-486. DOI: https://doi.org/10.1104/pp.124.2.485
Fleming, A. J. (2005). Formation of primordia and phyllotaxy. Current Opinion in Plant Biology, 8(1): 53-58. DOI 10.1016/j.pbi.2004.11.013
Fuglie, K. O., Adiyoga, W., Asmunati. R., Mahalaya, S., Suherman, R. (2006). Farm demand for quality potato seed in Indonesia. Agricultural Economics, 35(3): 257-266. DOI 10.1111/j.1574-0862.2006.00160.x
George, E. F., Hall, M. A., Klerk, G. J. D. (2008). Plant Tissue Culture Procedure - Background. In: George EF, Hall MA, Klerk GJD. (eds) Plant Propagation by Tissue Culture. Dordrecht: Springer. DOI 10.1007/978-1-4020-5005-3_1
Ha, C. M., Kim, G. T., Kim, B. C., Jun, J. H., Soh, M. S,, Ueno, Y.,
Machida, Y., Tsukaya, H., Nam, H. G. (2003). The Blade-on-Petiole 1 Gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis. Development, 130:161-172. DOI: 10.1242/Dev.00196
Hammarström, L., and Hammes-Schiffer, S. (2009). Artificial Photosynthesis and Solar Fuels. Acc. Chem. Res, 42(12): 1859-1860. DOI: 10.1021/ar900267k
Hartus, T. (2006). Usaha Pembibitan Kentang Bebas Virus. Jakarta: Penebar Swadaya. p 13-23
Hussain, A., Qarshi, I. A., Nazir, H., Ullah, I. 2012. Plant tissue culture: current status and opportunities, recent advances in plant in vitro culture, Dr. Annarita Leva (Ed.). InTech. DOI: 10.5772/50568. Available online: https://mts.intechopen.com/books/recent-advances-in-plant-in-vitro-culture/plant-tissue-culture-current-status-and-opportunities
Haverkort, A. J., and Verhagen, A. (2008). Climate Change and Its Repercussions for the Potato Supply Chain. Potato Res, 51: 223. DOI 10.1007/s11540-008-9107-0
Kac̆uráková, M., Capek, P., Sasinková, V., Wellner, N., Ebringerová, E. (2000). FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses. Carbohydrate Polymers, 43(2): 195-203. DOI: 10.1016/S0144-8617(00)00151-X
Kozuka, T., Horiguchi, G., Kim, G. T., Ohgishi, M., Sakai, T., Tsukaya, H. (2005). The Different Growth Responses of the Arabidopsis thaliana Leaf Blade and the Petiole during Shade Avoidance are regulated by Photoreceptors and Sugar. Plant and Cell Physiology, 46(1): 213–223. DOI 10.1093/pcp/pci016
Kristina, N. N., dan Syahid, S. F. (2012). Pengaruh air kelapa terhadap multiplikasi tunas in vitro, produksi rimpang. Jurnal Littri, 18(3): 125-134.
Marschner, H. (2002). Mineral Nutrition of Higher Plants. London: Academic Press. p 889.
Muthoni, J., Shimelis, H., Melis, R. 2013. Alleviating potato seed tuber shortage in developing countries: Potential of true potato seeds. Australian Journal of Crop Science, 7(12): 1946-1954
Oerke, E. C. (2005). Crop losses to pests. The Journal of Agricultural Science, 144(1): 31-43. DOI 10.1017/S0021859605005708
Oka, D. N. (2014). Coconut water medium increases the germination power of cucumber (Cucumis sativus L.) seed and the implementation in dormancy practicum. International Journal of Scientific Research and Education, 2(6): 1019-1028.
Perttunen, J., Nikinmaa, E., Lechowics, M. J., Sievänen, R., Messier, C. (2001). Application of the Functional-Structural Tree Model Lignum to Sugar Maple Saplings (Acer saccharum Marsh) Growing in Forest Gaps. Annals of Botany, 88(3): 471–481. DOI 10.1006/anbo.2001.1489
Prihatmanti, D., dan Mattjik, N. A. (2004). Penggunaan Zat Pengatur Tumbuh NAA (Naphtaleine Acetic Acid) dan BAP (6-Benzyl Amino Purine) serta Air Kelapa untuk Menginduksi Organogenesis Tanaman Anthurium (Anthurium andraeanum Linden Ex Andre). Bul. Agron, 32(1): 20-25
Reinhardt, D., Pesce, E. R., Stieger, P., Mandel, T., Baltensperger, K., Bennett, M., Traas, J., Friml, J., Kuhlemeier, C. (2003). Regulation of phyllotaxis by polar auxin transport. Nature, 426: 255-260. DOI 10.1038/nature02081
Resosudarmo, B. P. and Yamazaki, S. (2011). Training and Visit (T&V) Extension vs. Farmer Field School: The Indonesian Experience. The Australian National University. Working Paper No. 2011/01. Available online: http://rspas.anu.edu.au/economics/publications.php
Razdan, M. K. (2003). Introduction to Plant Tissue Culture. New Hamshire: Science Publishers. p 22-24
Rukmana, R. (2003). Aneka Olahan Kelapa. Yogyakarta: Kanisius. p 25-29
Scarpella, E., Barkoulas, M., Tsiantis, M. (2009). Control of Leaf and Vein Development by Auxin. Cold Spring Harbor Laboratory Press. DOI 10.1101/cshperspect.a001511
Seswita, D. (2010). Penggunaan air kelapa sebagai zat pengatur tumbuh pada multiplikasi tunas temulawak (Curcuma xanthorrhiza Roxb.) in vitro. Jurnal Penelitian Tanaman Industri, 16(4): 135-140. DOI: 10.21082/Littri.V16n4.2010.135%20-%20140
Sidhu, Y. (2010). In vitro micropropagation of medicinal plants by tissue culture. The Plymouth Student Scientist, 4(1): 432-449
Rahayu, S., Nadifah, F., Prasetyaningsih, Y. (2015). Jamur Kontaminan Pada Umbi Kentang. Biogenesis, 3(1): 28-32. DOI 10.24252/bio.v3i1.563
Thomasâ€Sharma, S., Abdurahman, A., Ali, S., Andradeâ€Piedra, J. L., Bao, S., Charkowski, A. O., Crook, D., Kadian, M., Kromann, P., Struik,
P. C., Torrance, L., Garret, K. A., Forbes, G. A. (2015). Seed degeneration in potato: the need for an integrated seed health strategy to mitigate the problem in developing countries. Plant Pathology, 65(1): 3-16. DOI 10.1111/ppa.12439
Timmer, C. P. (2003). Biotechnology and Food Systems in Developing Countries. The Journal of Nutrition, 133(11): 3319-3322. DOI 10.1093/jn/133.11.3319
Wu, G. (2009). Amino acids: metabolism, functions, and nutrition. Amino Acids, 37(1): 1-17. DOI 10.1007/s00726-009-0269-0
Downloads
Published
Issue
Section
License
Copyright (c) 2018 Scientiae Educatia

This work is licensed under a Creative Commons Attribution 4.0 International License.
LETTER OF COPYRIGHT AGREEMENT
I am the author of an article on Scientiae Educatia: Jurnal Pendidikan Sains in collaboration with the Perkumpulan Pendidik IPA Indonesia (PPII), the undersigned:
State that:
- My paper is the work itself, authentic, has never been published in journals and other publications;
- My paper is a work on original ideas / research, not the result of plagiarism;
- My paper was not published from the help of others, except on the recommendation of the editorial and reviewer board that was selected by this journal;
- My paper, there are no writings or opinions that I have written other than including the reference source in the bibliography;
- I give all my copyright of this paper to this journal include the right for managing and publication;
- I make this assignment surely. If there are distortion and untruth in this assignment, later, I will take responsibility as the current law.